Publications

Mapping and Modeling the Nanomechanics of Bare and Protein-Coated Lipid Nanotubes

Publié le 15/02/2020
n°DOI: 10.011031
Guillaume Lamour, Antoine Allard, Juan Pelta, Sid Labdi, Martin Lenz, Clément Campillo
Santé, Bien-être, Biotechnologies et Industrie

Membrane nanotubes are continuously assembled and disassembled by the cell to generate and dispatch transport vesicles, for instance, in endocytosis. While these processes crucially involve the ill-understood local mechanics of the nanotube, existing micromanipulation assays only give access to its global mechanical properties. Here we develop a new platform to study this local mechanics using atomic force microscopy (AFM). On a single coverslip we quickly generate millions of substrate-bound nanotubes, out of which dozens can be imaged by AFM in a single experiment.

 

Mapping and Modeling the Nanomechanics of Bare and Protein-Coated Lipid Nanotubes, Physical Review X 2020

Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore

Publié le 19/12/2019
n°10.1038/s41587-019-0345-2
Hadjer Ouldali, Kumar Sarthak, Tobias Ensslen, Fabien Piguet, Philippe Manivet, Juan Pelta, Jan C. Behrends, Aleksei Aksimentiev, Abdelghani Oukhaled
Santé, Bien-être, Biotechnologies et Industrie

Efforts to sequence single protein molecules in nanoporeshave been hampered by the lack of techniques with sufficient sensitivity to discern the subtle molecular differences among all twenty amino acids. Here we report ionic current detection of all twenty proteinogenic amino acids in an aerolysin nanopore with the help of a short polycationic carrier. Application of molecular dynamics simulations revealed that the aerolysin nanopore has a built-in single-molecule trap that fully confines a polycationic carrier-bound amino acid inside the sensing region of the aerolysin.

 

Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore, Nature Biotechnology 2019

Photonic titanium dioxide film obtained from hard template with chiral nematic structure for environmental application

Publié le 02/09/2019
n°10.1016/j.cattod.2019.01.030
Chunyu Li, Erwan Paineau, François Brisset, Sylvain Franger, Christophe Colbeau-Justin, Mohamed N.Ghazzal
Sciences environnementales et Sécurité

In the present work, mesoporous TiO2 with a photonic structure was elaborated using cellulose nanocrystals (CNCs) as a biotemplate by two-step hard template methods.

This strategy enables to replicate the chiral nematic (CN) structure of the photonic films (biotemplate) in TiO2 films.

A series of iridescent CNCs films with different weight ratios of silica/CNCs composite photonic films were prepared via evaporation induced self-assembly (EISA) method.

 

Photonic titanium dioxide film obtained from hard template with chiral nematic structure for environmental application, Catalysis Today 2019

Enhanced photogenerated charge carriers and photocatalytic activity of biotemplated mesoporous TiO2 films with a chiral nematic structure

Publié le 06/06/2019
n°10.1021/acs.chemmater.9b01465
Getaneh Diress Gesesse, Chunyu Li, Erwan Paineau, Youssef Habibi, Hynd Remita, Christophe Colbeau-Justin, Mohamed Nawfal-Ghazzal
Energies renouvelables

Improving the photogeneration and the lifetime of charge carriers associated with light harvesting is among the main challenges facing materials for photocatalysis. We report here the synthesis of mesoporous TiO2 containing a replica of a chiral nematic structure (CNS) as a photocatalyst with improved light harvesting and photogenerated charge carriers under UV illumination. The CNS of cellulose nanocrystal photonic films, obtained by an evaporation-induced self-assembly method, were successfully transferred into an inorganic TiO2 film by sol–gel mineralization of the biotemplate.

 

Enhanced photogenerated charge carriers and photocatalytic activity of biotemplated mesoporous TiO2 films with a chiral nematic structure, American Chemical Society

Aerolysin, a powerful protein sensor for fundamentalstudies and development of upcoming applications

Publié le 23/03/2019
n°10.1021/acssensors.8b01636
Benjamin Cressiot, Hadjer Ouldali, Manuela Pastoriza-Gallego, F. Gisou van der Goo, Laurent Bacri, Juan Pelta
Sciences environnementales et Sécurité

Nanopore electrical approach is a breakthrough in single molecular level detection of particles as small  as  ions,  and  complex  as  biomolecules.

This  technique  can  be  used  for  molecule  analysis, and characterization as well as for the understanding of confined medium dynamics in chemical or biological reactions.

Altogether, the information obtained from these kinds of experiments will allow to address challenges in a variety of biological fields. The sensing, design and manufacture ofnanopores is crucial to obtain these objectives.

 

Aerolysin, a powerful protein sensor for fundamentalstudies and development of upcoming applications, HAL Archives 2019

A Robust Energy-Efficient Metal-Organic Framework Adsorbent for Refrigeration

Publié le 22/10/2018
n°DOI: 10.1038/s41560-018-0261-6
Sujing Wang, Ji Sun Lee, Mohammad Wahiduzzaman, Jaedeuk Park, Mégane Muschi, Charlotte Martineau-Corcos, Antoine Tissot, Kyung Ho Cho, Jérôme Marrot, William Shepard, Guillaume Maurin, Jong-San Chang, Christian Serre
Energies renouvelables

The discovery of more-efficient and stable water adsorbents for adsorption-driven chillers for cooling applications remains a challenge due to the low working capacity of water sorption, high regeneration temperature, low energy efficiency under given operating conditions and the toxicity risk of harmful working fluids for the state-of-the-art sorbents. Here we report the water-sorption properties of a porous zirconium carboxylate metal–organic framework, MIP-200, which features S-shaped sorption isotherms, a high water uptake of 0.39 g g−1 below P/P0 = 0.25, facile regeneration and stable cycling, and most importantly a notably high coefficient of performance of 0.78 for refrigeration at a low driving temperature (below 70 °C).

 

A Robust Energy-Efficient Metal-Organic Framework Adsorbent for Refrigeration, Nature Energy 2018