Efforts to sequence single protein molecules in nanoporeshave been hampered by the lack of techniques with sufficient sensitivity to discern the subtle molecular differences among all twenty amino acids. Here we report ionic current detection of all twenty proteinogenic amino acids in an aerolysin nanopore with the help of a short polycationic carrier. Application of molecular dynamics simulations revealed that the aerolysin nanopore has a built-in single-molecule trap that fully confines a polycationic carrier-bound amino acid inside the sensing region of the aerolysin.
Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore, Nature Biotechnology 2019
In the present work, mesoporous TiO2 with a photonic structure was elaborated using cellulose nanocrystals (CNCs) as a biotemplate by two-step hard template methods.
This strategy enables to replicate the chiral nematic (CN) structure of the photonic films (biotemplate) in TiO2 films.
A series of iridescent CNCs films with different weight ratios of silica/CNCs composite photonic films were prepared via evaporation induced self-assembly (EISA) method.
Improving the photogeneration and the lifetime of charge carriers associated with light harvesting is among the main challenges facing materials for photocatalysis. We report here the synthesis of mesoporous TiO2 containing a replica of a chiral nematic structure (CNS) as a photocatalyst with improved light harvesting and photogenerated charge carriers under UV illumination. The CNS of cellulose nanocrystal photonic films, obtained by an evaporation-induced self-assembly method, were successfully transferred into an inorganic TiO2 film by sol–gel mineralization of the biotemplate.
Nanopore electrical approach is a breakthrough in single molecular level detection of particles as small as ions, and complex as biomolecules.
This technique can be used for molecule analysis, and characterization as well as for the understanding of confined medium dynamics in chemical or biological reactions.
Altogether, the information obtained from these kinds of experiments will allow to address challenges in a variety of biological fields. The sensing, design and manufacture ofnanopores is crucial to obtain these objectives.
The confined dynamics of water molecules inside a pore involves an intermittence between adsorption steps near the interface and surface diffusion and excursions in the pore network.
Depending on the strength of the interaction in the layer(s) close to the surface and the dynamical confinement of the distal bulk liquid, exchange dynamics can vary significantly. The average time spent in the surface proximal region (also called the adsorption layer) between a first entry and a consecutive exit allows estimating the level of ‘nanowettablity’ of water. As shown in several seminal works, NMRD is an efficient experimental method to follow such intermittent dynamics close to an interface.
There are still unmet needs in finding new technologies for biomedical diagnostic and industrial applications. A technology allowing the analysis of size and sequence of short peptide molecules of only few molecular copies is still challenging. The fast, low-cost and label-free single-molecule nanopore technology could be an alternative for addressing these critical issues.