Development of porous scaffolds with advanced electromechanical properties for cardiac tissue engineering.

Post-doctorale allowance, AAP 2019

Team: Institut de Biologie Paris Seine-B2A, Team Stem Cells, Cardiovascular Pathophysiology and Biotherapies.

Project leader: Onnik Agbulut

Résumé :

It is a project that aims to develop new porous materials that can mimic the extracellular matrix of cardiac tissue. Recent advances in nanotechnology have greatly improved our capacities to mimic the extracellular matrix.  Among them, electrospinning is a well-known method for being easy to process and cost‑effective. Taking advantage of the capabilities that electrospinning can offer, the focus will be in the creation of an aligned network of fibers for resembling the native structure of cardiac tissue. In our lab we are equipped with an electrospinning system that has the component for the fabrication of aligned fibers, which was co-financed by the DIM Respore. The development of the 3D scaffold will be based on combining the ECM mimicking with adequate electromechanical properties. Therefore, it is important to evaluate the forces that the developed scaffolds apply to cardiomyocytes. This part of the project will be realized, in collaboration with the Université d’Evry Val d’Essonne, LAMBE – UMR 8587, using the method of atomic force microscopy (AFM). AFM can provide with useful insights of the mechanical properties of the scaffolds and of living cells in real time and at nanometer resolution.